Interplay of long-chain tetrazine derivatives and biomembrane components at the air–water interface

Tetrazine (Tz) is an emerging bioorthogonal ligand that expected to have applications (e.g., bioimaging) in chemistry and chemical biology. In this review, we highlight the interactions of reduced tetrazine (rTz) derivatives insoluble aqueous media with biological membrane constituents or their related lipids, such as dipalmitoyl-phosphatidylcholine, dipalmitoyl-phosphatidylethanolamine, dipalmitoyl-phosphatidylglycerol, palmitoyl-sphingomyelin, cholesterol Langmuir monolayer state at air–water interface. The two-component interaction was thermodynamically elucidated by measuring surface pressure (π) molecular area (A) isotherms. miscibility between two components analyzed using excess Gibbs energy mixing two-dimensional phase diagram. behavior binary monolayers studied Brewster angle, fluorescence, atomic force microscopy. This study discusses affinities rTz moieties for hydrophilic groups lipids used.